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Bacterial Adaptation & Response Networks

Eltis Lab Publications

2010

Okamoto S, van Petegem F, Patrauchan MA, and Eltis LD. 2010. AnhE, a metallochaperone involved in the maturation of a cobalt-dependent nitrile hydratase. J Biol Chem. doi: 10.1074/jbc.M110.10922

Dresen C, Lin L, D’Angelo I, Tocheva EI, Strynadka N and Eltis LD. 2010. A flavin-dependent monooxygenase from mycobacterium tuberculosis involved in cholesterol catabolism. J Biol Chem. doi:10.1074/jbc.M109.09902

Huitema C and Eltis LD. 2010. A fluorescent protein-based biological screen of proteinase activity. J Biomol Screen 15, 224-9.

Mathieu J, Mohn WW, Eltis LD, Leblanc J, Stewart G, Dresen C, Okamoto K and Alvarez PJ. 2010. 7-Ketocholesterol catabolism by Rhodococcus jostii RHA1. Appl Environ Microbiol. 76, 352-5.

Lack NA, Yam KC, Lowe ED, Horsman GP, Owen RL, Sim E and Eltis LD. 2010. Characterization of a C-C hydrolase from Mycobacterium tuberculosis involved in cholesterol metabolism. J Biol Chem. 285, 434-43.

2009

Capyk JK, Kalscheuer R, Stewart GR, Liu J, Kwon H, Zhao R, Okamoto S, Jacobs WR Jr, Eltis LD and Mohn WW. 2009. Mycobacterial cytochrome P450 125 (Cyp125) catalyzes the terminal hydroxylation of C27-steroids. J Biol Chem. PMID: 19846551

Rosloniec KZ, Wilbrink MH, Capyk JK, Mohn WW, Ostendorf M, wan der Geize R, Dijkhuizen L and Eltis LD. (2009). Cytochrome P450 125 (Cyp125) catalyzes C26-hydroxylation to initiate sterol side-chain degradation in Rhodococcus jostii RHA1. 2009. Mol Microbiol. PMID: 19843222

Yam KC, D’Angelo I, Kalscheuer R, Zhu H, Wang JX, Snieckus V, Ly LH, Converse PJ, Jacobs WR Jr, Strynadka N, and Eltis LD. 2009. Studies of a ring-cleaving dioxygenase illuminate the role of cholesterol metabolism in the pathogenesis of Mycobacterium tuberculosis. PLoS Pathog. 5, e1000344.

Capyk JK, D’Angelo I, Strynadka N, and Eltis LD. 2009. Characterization of 3-ketosteroid 9alpha-hydroxylase, a Rieske oxygenase in the cholesterol degradation pathway of Mycobacterium tuberculosis. J. Biol. Chem., 284, 9937-46.

2008

Mohn WW, van der Geize R, Stewart GR, Okamoto S, Liu J, Dijkhuizen L and Eltis LD. The actinobacterial mce4 locus encodes a steroid transporter. J. Biol. Chem. 283, 35368-74.

Brown EN, Friemann R, Karlsson A, Parales JV, Couture MM, Eltis LD, and Ramaswamy S. 2008. Determining Rieske cluster reduction potentials. J. Biol. Inorg. Chem. 13, 1301-13.

Zhang J, Huitema C., Niu C, Yin J, James MN, Eltis LD, and Vederas JC. 2008. Aryl methylene ketones and fluorinated methylene ketones as reversible inhibitors for severe acute respiratory syndrome (SARS) 3C-like proteinase. Bioorg. Chem. 36, 229-40.

Huitema C, Zhang J, Yin J, James MN, Vederas JC, and Eltis LD. 2008. Heteroaromatic ester inhibitors of hepatitis A virus 3C proteinase: evaluation of mode of action. Bioorg. Med. Chem. 16, 5761-77.

Master ER, Agar NY, Gomez-Gil, Powlowski JB, Mohn WW, and Eltis LD. 2008. Biphenyl dioxygenase from an arctic isolate is not cold adapted. Appl. Environ. Microbiol. 74, 2908-11.

Poetsch A, Schlusener D, Florizone C, Eltis LD, Menzel C, Rogner M, Steinert K, and Roth U. 2008. Improved identification of membrane proteins by MALDI-TOF MS/MS using vacuum sublimated matrix spots on an ultraphobic chip surface. J. Biomol. Tech. 2, 129-38.

Tocheva EI, Eltis LD, and Murphy ME. 2008. Conserved active site residues limit inhibition of a copper-containing nitrite reductase by small molecules. Biochemistry 47, 4452-60.

Eltis LD and Withers SG. 2008. Nature teaches but can be bettered. Curr. Opin. Chem. Biol. 12, 115-7.

Smith DJ, Patrauchan MA, Florizone C, Eltis LD, and Mohn WW. 2008. Distinct roles for two CYP226 family cytochromes P450 in abietane diterpenoid catabolism by Burkholderia xenovorans LB400. J. Bacteriol. 190, 1575-83.

Patrauchan MA, Florizone C, Eapen S, Gomez-Gil L, Sethuraman B, Fukuda M, Davies J, Mohn WW, and Eltis LD. 2008. Roles of ring-hydroxylating dioxygenases in styrene and benzene catabolism in Rhodococcus jostii RHA1. J. Bacteriol. 190, 37-47.

Lack N, Lowe ED, Liu J, Eltis LD, Noble ME, Sim E, and Westwood IM. 2008. Structure of HsaD, a steroid-degrading hydrolase, from Mycbacterium tuberculosis. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 64, 2-7.

McLeod MP, Eltis LD. Genomic insights into the aerobic degradation of organic pollutants. In Microbial Biodegradation: genomics and molecular biology. (Ed. Diaz), 2008, Horizon Scientific Press.

2007

Sharp JO, Sales CM, LeBlanc J, Liu J, Wood TK, Eltis LD, Mohn WW, and Alvarez-Cohen L. 2007. An inducible propane monooxygenase is responsible for N-nitrosodimethylamine degradation by Rhodococcus sp. RHA1. Appl. Environ. Microbiol., 73, 6930-8.

Hekmat O, Florizone C, Kim Y-W, Eltis LD, Warren RAJ, and Withers SG. 2007. Specificity fingerprinting of retaining -1,4-glycanases in Cellulomonas fimi secretome using two fluorescent mechanism-based probes. Chem. Bio. Chem. 8, 2125-32.

Okamoto S, and Eltis LD. 2007. Purification and characterization of a novel nitrile hydratase from Rhodococcus sp. RHA1. Mol. Micro. 65, 828-838.

Yin J, Niu C, Cherney MM, Zhang J, Huitema C, Eltis LD, Vederas JC and James MNG. 2007. A mechanistic view of enzyme inhibition and peptide hydrolysis in the active site of the SARS-CoV 3C-like peptidase. J. Mol. Biol. 371, 1060-74.

Horsman GP, Bhowmik S, Seah SYK, Kumar P, Bolin JT, and Eltis LD. 2007. The tautomeric half-reaction of BphD, a C-C bond hydrolase: kinetic and structural evidence supporting a key role for histidine 265 of the catalytic triad. J. Biol. Chem. 282, 19894-904.

Gómez L, Kumar P, Barriault D, Sylvestre M, Bolin JT, and Eltis LD. 2007. Characterization of biphenyl dioxygenase of Pandoraea pnomenusa B-356 as a potent polychlorinated biphenyl-degrading enzyme. J. Bacteriol. 189, 5705-5715.

Nerdinger S, Kendall C, Cai X, Marchart R, Riebel P, Johnson MR, Yin C-F, Haenaff N, Eltis LD, and Snieckus V. 2007. Combined directed ortho metalation – Suzuki-Miyaura cross-coupling strategies. Regiospecific synthesis of chlorodihydroxybiphenyls and Polychlorinated Biphenyls. J. Org. Chem. 72, 5960-7.

Seah SYK, Denis G, Ke J, Fortin PD, Horsman GP, Whiting C, and Eltis LD. 2007. Cloning, sequencing and characterization of a C-C hydrolase from Sphingomonas wittichii RW1 with novel specificities towards chlorinated HOPDAs. J. Bacteriol., 189, 4038-4045.

Zhang J, Pettersson HI, Huitema C, Niu C, Yin J, James MNG, Eltis LD, and Vederas JC. 2007. Design, synthesis and evaluation of inhibitors for Severe Acute Respiratory Syndrome 3C-like protease based on phthalhydrazide ketones or heteroaromatic esters. J. Med. Chem. 50, 1850-64.

Lee T-W, Cherney MM, Liu J, James KE, Powers JC, Eltis LD, James MNG. 2007. Crystal structures reveal an induced-fit binding of a substrate-like aza-peptide epoxide to SARS coronavirus main peptidase. J. Mol. Biol. 366, 916-32.

Van der Geize R, Yam K, Heuser T, Wilbrink MH, Hara H, Anderton MC, Sim E, Davies JE, Dijkhuizen L, Mohn WW, and Eltis LD. 2007. A gene cluster encoding cholesterol catabolism in a soil actinomycete provides insight into Mycobacterium tuberculosis survival in macrophages. Proc. Natl. Acad. Sci. USA, 104, 1947-1952.

Hara H, Eltis LD, Davies J, and Mohn WW. 2007. Transcriptomic analysis reveals a bifurcated terephthalate degradation pathway in Rhodococcus sp. RHA1. J. Bacteriol. 189, 1641-7.

Kappl R, Eltis LD, Caspersen MB, Christensen HEM, and Hüttermann J. 2007. Site-specific oxidation of the [Fe4S4] cubanes in HiPIPs as probed by EPR and orientation selective proton ENDOR spectroscopy: Ectothiorhodospira halophila I vs. Rhodocyclus tenuis. Appl. Magn. Reson. 31, 483-507.

McLeod MP, Eltis LD. Genomic insights into the aerobic degradation of organic pollutants. In Microbial Biodegradation: genomics and molecular biology. (Ed. Diaz), ã2007, Horizon Scientific Press.

2006

McLeod MP, Warren RL, Hsiao WWL, Araki N, Myhre M, Fernandes C, Miyazawa D, Wong W, Lillquist AL, Wang D, Dosanjh M, Hara H, Petrescu A, Morin RD, Yang G, Stott JM, Schein JE, Shin H, Smailus D, Siddiqui AS, Marra MA, Jones SJM, Holt R, Brinkman FSL, Miyauchi K, Fukuda M, Davies JE, Mohn WW, and Eltis LD. 2006. The complete genome of Rhodococcus sp. RHA1 provides insights into a catabolic powerhouse. Proc. Natl. Acad. Sci. USA 103, 15582-15587.

Tocheva EI, Fortin PD, Eltis LD, Murphy MEP. 2006. Ternary complexes of BphK, a bacterial GST that reductively dechlorinates PCB metabolites. J. Biol. Chem. 281, 30933-40.

Horsman GP, Ke J, Dai S, Seah SYK, Bolin JT, Eltis LD. 2006. Kinetic and structural insight into the mechanism of BphD, a C-C bond hydrolase from the biphenyl degradation pathway. Biochemistry, 45, 11071-11086.

Couture MMJ, Martin VJJ, Mohn WW, Eltis LD. 2006. Characterization of DitA3, the [Fe3S4] ferredoxin of an aromatic ring-hydroxylating dioxygenase from a diterpenoid degrading microorganism. Biochim. Biophys. Acta 1764, 1462-9.

Gonçalves ER, Hara H, Miyazawa D, Davies JE, Eltis LD, Mohn WW. 2006. Transcriptomic Assessment of Isozymes in the Biphenyl Pathway of Rhodococcus sp. RHA1. Appl. Environ. Microbiol. 72, 6183-6193.

Yin J, Cherney MM, Bergmann EM, Zhang J, Huitema C, Pettersson H, Eltis LD, Vederas JC, James MNG. 2006. An episulfide cation (thiiranium ring) trapped in the active site of HAV 3C proteinase inactivated by peptide-based ketone inhibitors. J. Mol. Biol. 361, 673-686.

Fortin PD, Horsman GP, Yang HM, Eltis LD. 2006. A glutathione S-transferase improves the PCB-degrading capabilities of the bph pathway. J. Bacteriol. 188, 4424-4430.

Anderton MC, Bhakta S, Besra GS, Jeavons P, Eltis LD, Sim E. 2006. Characterisation of the putative operon containing arylamine N-acetyltransferase (nat) in Mycobacterium bovis BCG. Mol. Microbiol. 59, 181-192.

Vaillancourt FH, Bolin JT, and Eltis LD. 2006. The ins and outs of ring-cleaving dioxygenases. Crit. Rev. Biochem. Mol. Biol. 41, 241-267.

2005

Denef VJ, Klappenbach JA, Patrauchan MA, Florizone C, Rodrigues JLM, Tsoi TV, Verstraete W, Eltis LD, Tiedje JM. 2005. Genetic and genomic insights into the role of benzoate-catabolic pathway redundancy in Burkholderia xenovorans LB400. Appl. Environ. Microbiol. 72, 585-595.

Fortin PD, MacPherson I, Neau DB, Bolin JT, and Eltis LD. 2005. Directed evolution of a ring-cleaving dioxygenase for polychlorinated biphenyl degradation. J. Biol. Chem. 280, 42307-14.

Horsman GP, Jirasek A, Vaillancourt FH, Barbosa CJ, Jarzecki AA, Xu C, Mekmouche Y, Spiro TG, Lipscomb JD, Blades MW, Turner RFB, Eltis LD. 2005. Spectroscopic studies of the anaerobic enzyme-substrate complex of catechol 1,2-dioxygenase. J. Amer. Chem. Soc., 127, 16882-91.

Denef VJ, Patrauchan MA, Florizone C, Park J, Tsoi TV, Verstraete W, Tiedje JM, Eltis LD. 2005. Growth substrate and phase specific expression of biphenyl, benzoate and C1 metabolic pathways in Burkholderia xenovorans LB400. J. Bacteriol. 187, 7996-8005.

Vaillancourt FH, Fortin PD, Labbé G, Drouin NM.,3, Karim Z, Agar NYR, Eltis LD. 2005. Molecular basis for the substrate selectivity of bicyclic and monocyclic extradiol dioxygenases. Biochem. Biophys. Res. Commun. 338, 215-222.

Lee T-W, Cherney MM, Huitema C, Liu J, Eltis LD, James KE, Powers JC, James MNG. 2005. Crystal structures of the main protease from the SARS coronavirus inhibited by a substrate-like aza-peptide epoxide. J. Mol. Biol. 353, 1137-51.

Navarro-Llorens JM, Patrauchan MA, Stewart GR, Davies J, Eltis LD, Mohn WW. 2005. The catabolism of phenylacetic acid in Rhodococcus RHA1. J. Bacteriol. 187, 4497-4504.

Patrauchan MA, Florizone C, Dosanjh M, Mohn WW, Davies J, Eltis LD. 2005. Catabolism of benzoate and phthalate in Rhodococcus sp. Strain RHA1: redundancies and convergence. J. Bacteriol. 187, 4050-4063.

Veldhuizen EJA, Vaillancourt FH, Whiting CJ, Hsiao MMY, Gingras G, Xiao Y, Tanguay RM, Boukouvalas J, Eltis LD. 2005. Steady-State Kinetics and Inhibition of Anaerobically Purified Human Homogentisate 1,2-Dioxygenase. Biochem. J., 386, 305-314.

Fortin PD, Lo ATF, Haro MA, Kaschabek SR, Reineke W, Eltis LD. 2005. Evolutionarily Divergent Extradiol Dioxygenases Possess Higher Specificities for PCB Metabolites. J. Bacteriol. 187, 415-421.

2004

Jain RP, Pettersson HI, Zhang J, Aull KD, Fortin PD, Huitema C, Eltis LD, Parrish JC, James MNG, Wishart DS, Vederas JC. 2004. Synthesis and Evaluation of Keto-Glutamine Analogs as Potent Inhibitors of SARS 3CLpro. J. Med Chem., 47, 6113-6116.

Warren R, Hsiao WWL, Kudo H, Myhre M, Dosanjh M, Petrescu A, Kobayashi H, Shimizu S, Miyauchi K, Masai E, Yang G, Stott JM, Schein JE, Shin H, Khattra J, Smailus D, Butterfield YS, Siddiqui A, Holt R, Marra MA, Jones SJM, Mohn WW, Brinkman FSL, Fukuda M, Davies J, Eltis LD. 2004. Functional Characterization of a Catabolic Plasmid from PCB-Degrading Rhodococcus sp. RHA1. J. Bacteriol., 186, 7783-7795.

Blanchard JE, Elowe NH, Fortin PD, Huitema C, Cechetto JD, Eltis LD, Brown ED. 2004. High throughput screening identifies inhibitors of the SARS coronavirus main proteinase. Chem. Biol., 11, 1445-1453.

Dey A, Glaser T, Couture MMJ, Eltis LD, Holm RH, Hedman B, Hodgson KO, and Solomon EI. 2004. Ligand K-edge X-ray Absorption Spectroscopy of [Fe4S4]1+,2+,3+ Clusters: Changes in Bonding and Electronic Relaxation upon redox. J. Amer. Chem. Soc., 126, 8320-8328.

Jirasek A, Horsman GP, Vaillancourt FH, Barbosa CJ, Eltis LD, Blades MW and Turner RFB. 2004. Probing enzyme-substrate interactions using fiber-optic ultraviolet resonance Raman spectroscopy. Am. Pharm. Rev., 7(2), 49-53.

Vaillancourt FH, Bolin JT, and Eltis LD. Ring-cleavage dioxygenases. In The Pseudomonads Vol III. Biosynthesis of Macromolecules and Molecular metabolism (Ed. Ramos), pp. 359-95, 2004, Kluwer Academic/Plenum Publishers, New York.

2003

Zu Y, Couture MMJ, Kolling DRJ, Crofts AR, Eltis LD, Fee JA, and Hirst J. 2003. The reduction potentials of Rieske clusters: the importance of the coupling between oxidation state and histidine protonation state. Biochemistry, 42, 12400-12408.

Davis MI, Wasinger EC, Decker A, Pau MYM, Vaillancourt FH, Bolin JT, Eltis LD, Hedman B, Hodgson KO, and Solomon EI. 2003. Spectroscopic and electronic structure studies of 2,3-dihydroxybiphenyl 1,2-dioxygenase: O2 reactivity of the non-heme ferrous site in extradiol dioxygenases. J. Amer. Chem. Soc., 125. 11214-11227.

Ge Y, and Eltis LD. 2003. Characterization of hybrid toluate and benzoate dioxygenases. J. Bacteriol., 185, 5333-5341.

Vaillancourt FH, Haro M-A, Drouin NM, Karim Z, Maaroufi H, Eltis LD. 2003. Characterization of extradiol dioxygenases from a PCB-degrading strain that possess higher specificities for chlorinated metabolites. J. Bacteriol., 185, 1253-1260

2002

Dai S, Vaillancourt FH, Maaroufi H, Drouin NM, Neau DB, Snieckus V, Bolin JT, and Eltis LD. 2002. Dioxygenase-catalyzed cleavage of PCB metabolites: identification and analysis of inhibitory species. Nature Struct. Biol. 9, 334-339

Ge Y, Vaillancourt FH, Agar NYR, Eltis LD. 2002. The reactivity of toluate dioxygenase with substituted benzoates and dioxygen. J. Bacteriol., 184, 4096-4103.

Barbosa CJ, Vaillancourt FH, Eltis LD, Blades MW, Turner RFB. 2002. The power distribution advantage of fiber optic coupled Ultraviolet resonance Raman spectroscopy for biological samples. J. Raman Spec., 33, 503-510.

Vaillancourt FH, Barbosa CJ, Spiro TG, Bolin JT, Blades MW, Turner RFB, and Eltis LD. 2002. Definitive evidence for monoanionic binding of 2,3-dihydroxybiphenyl to 2,3-dihydroxybiphenyl 1,2-dioxygenase from UV resonance Raman spectroscopy, UV/Vis absorption spectroscopy, and crystallography. J. Amer. Chem. Soc. 124:2485-96.

Vaillancourt FH, Labbé G, Drouin NM, Fortin PD, Eltis LD. 2002. The mechanism of the suicide inhibition of 2,3-dihydroxybiphenyl 1,2-dioxygenase by catecholic substrates. J. Biol. Chem., 277, 2019-2027.

2001

Vaillancourt FH, Barbosa CJ, Spiro TG, Bolin, JT, Blades MW, Turner RFB, and Eltis LD. 2001. Definitive evidence for monoanionic binding of 2,3-dihydroxybiphenyl to 2,3-dihydroxybiphenyl 1,2-dioxygenase from UV resonance Raman and UV/Vis absorption spectroscopies. J. Amer. Chem. Soc., 124:2485-2496.

Vaillancourt FH, Labbé G, Drouin NM, Fortin PD, and Eltis LD. 2001.The mechanism of the suicide inhibition of 2,3-dihydroxybiphenyl 1,2-dioxygenase by catecholic substrates. J. Biol. Chem., 277:2019-2027.

Seah SYK, Labbé G, Kaschabek SR, Reifenrath F, Reineke W, and Eltis LD. 2001. Comparative specificities of two evolutionarily divergent hydrolases involved in the microbial degradation of PCBs. J. Bacteriol., 183:1511-1516.

Couture MMJ, Colbert CL, Babini E, Rosell FI, Mauk AG, Bolin JT, and Eltis LD. 2001. Characterization of BphF, a Rieske-type ferredoxin with a low reduction potential. Biochemistry, 40:84-92.

2000

Colbert CL, Couture MMJ, Eltis LD, and Bolin JT. 2000. A cluster exposed: structure of the Rieske ferredoxin from biphenyl dioxygenase and the redox properties of Rieske FeS proteins. Structure Fold. Des., 8:1267-1278.

Seah SYK, Labbé G, Nerdinger S, Johnson MR, Snieckus V, and Eltis LD. 2000. Identification of a key determinant in the microbial degradation of PCBs. J. Biol. Chem., 275:15701-15708.

Imbeault NYR, Powlowski JB, Colbert CL, Bolin JT, and Eltis LD. 2000. Steady-state kinetic characterization and crystallization of a PCB-transforming dioxygenase. J. Biol. Chem., 275:12430-12437.

1999

Nerdinger S, Kendall C, Marchhart R, Riebel P, Johnson MR, Yin C-F, Eltis LD, and Snieckus V. 1999. Directed ortho metalation – Suzuki-Miyaura cross coupling connections: regiospecific synthesis of all isomeric chloro dihydroxybiphenyls to study the microbial degradation of PCBs. Chem. Commun., 22:2259-2260.

Babini E, Borsari M, Capozzi F, Eltis LD, and Luchinat C. 1999. Experimental evidence for the role of buried polar groups in determining the reduction potential of metalloproteins: the S79P variant of Chromatium vinosum HiPIP. J. Biol. Inorg. Chem., 4:692-700.

Couture MMJ, Auger M, Rosell FI, Mauk AG, Boubour E, Lennox RB, and Eltis LD. 1999. Investigation of the role of a surface patch in the self-association of Chromatium vinosum High potential iron-sulfur protein. Biochim. Biophys. Acta, 1433:159-169.

1998

Vaillancourt FH, Han S, Fortin PD, Bolin JT, and Eltis LD. 1998. Molecular basis for the stabilization and inhibition of 2,3-dihydroxybiphenyl 1,2-dioxygenase by t-butanol. J. Biol. Chem., 273:34887-34895.

Barriault D, Durand J, Maaroufi H, Eltis LD, and Sylvestre M. 1998. Degradation of PCB metabolites by naphthalene-catabolizing enzymes. Appl. Environ. Microbiol., 64:4637-4642.

Wan L, Twitchett MB, Eltis LD, Mauk AG, and Smith M. 1998. In vitro evolution of horse heart myoglobin to increase peroxidase activity. Proc. Natl. Acad. Sci. USA., 95:12825-12831.

Seah SYK, Terracina G, Bolin JT, Riebel P, Snieckus V, and Eltis LD. 1998. Purification and preliminary characterization of a serine hydrolase involved in the microbial degradation of PCBs. J. Biol. Chem., 273:22943-22949.

Bergdoll M, Eltis LD, Cameron AD, Dumas P, and Bolin JT. 1998. All in the family: structural and evolutionary relationships among three modular proteins with diverse functions and variable assembly. Protein Science, 7:1661-1670 (includes cover photo).

Hülsmeyer M, Hecht H-J, Niefind K, Hofer B, Eltis LD, Timmis KN, and Schomburg D. 1998. Crystal structure of cis-biphenyl-2,3-dihydrodiol-2,3-dehydrogenase from a PCB-degrader at 2.0 Å resolution. Protein Science, 7:1286-1293.

1997

Gauthier M, Brochu D, Eltis LD, and Vadeboncoeur C. 1997. Replacement of isoleucine 47 by threonine in the HPr protein of Streptococcus salivarius abrogates the preferential metabolism of glucose and fructose over lactose and melibiose but does not prevent the phosphorylation of HPr on serine 46. Mol. Microbiol., 25:695-705.

Hunter CL, Lloyd E, Eltis LD, Rafferty SP, Lee H, Smith M, and Mauk AG. 1997. The role of heme propionates in the interaction of heme with apomyoglobin and apocytochrome b5. Biochemistry, 36:1010-1017.

1996

Eltis LD, and Bolin JT. 1996. Evolutionary relationships among extradiol dioxygenases. J. Bacteriol., 178:5930-5937.

Bertini I, Couture MMJ, Donaire A, Eltis LD, Felli IC, Luchinat C, Piccioli M, and Rosato A. 1996. The solution structure refinement of the paramagnetic reduced HiPIP I from E. halophila: using stable isotope labeling and nuclear relaxation. Eur. J. Biochem., 241:440-452.

Bentrop D, Bertini I, Capozzi F, Dikiy A, Eltis LD, and Luchinat C. 1996. Three-dimensional structure of the reduced C77S mutant of the Chromatium vinosum high potential iron-sulfur protein through nuclear magnetic resonance: comparison with the solution structure of the wild-type protein. Biochemistry, 35:5928-5936.

Bertini I, Borsari M, Bosi M, Eltis LD, Felli I, Luchinat C, and Piccioli M. 1996. The influence of a surface charge on the electronic and steric structure of a High Potential Iron-Sulfur Protein. J. Biol. Inorg. Chem., 1:257-263.

Babini E, Bertini I, Borsari M, Capozzi F, Dikiy A, Eltis LD, and Luchinat C. 1996. A serine to cysteine ligand mutation in the high potential iron-sulfur protein from Chromatium vinosum provides insight into the electronic structure of the [4Fe-4S] cluster. J. Amer. Chem. Soc., 118:75-80.

1995

Iwagami S, Creagh AL, Haynes CA, Borsari M, Felli I, Piccioli M, and Eltis LD. 1995. Investigation into the role of a conserved tyrosine residue in high potential iron sulfur proteins. Protein Science, 4:2562-2572.

Han S, Eltis LD, Timmis KN, Muchmore SW, and Bolin JT. 1995. Crystal structure of a biphenyl-cleaving extradiol dioxygenase from a PCB-degrading pseudomonad. Science, 270:976-980.

Bertini I, Eltis LD, Felli I, Kastrau DHW, Luchinat C, and Piccioli M. 1995. The solution structure of oxidized HiPIP I from Ectothiorhodospira halophila: can NMR spectroscopy by used to probe rearrangements associated with electron transfer processes? Chem. Eur. J., 1:598-607.

Bertini I, Capozzi F, Eltis LD, Felli I, Luchinat C, and Piccioli M. 1995. Sequence specific assignment of ligand cysteine protons of oxidized, recombinant HiPIP I from Ectothiorhodospira halophila. Inorgan. Chem., 34:2516-2523.

Winkler J, Eltis LD, Dwyer DF, and Rhode M. 1995. The tetrameric structure and cellular location of catechol 2,3-dioxygenase. Arch. Microbiol., 163:65-69.

1994

Banci L, Bertini I, Eltis LD, Felli I, Kastrau DHW, Luchinat C, Piccioli M, Pierattelli R, and Smith M. 1994. The three dimensional structure of the paramagnetic HiPIP I from E. halophila through nuclear magnetic resonance. Eur. J. Biochem., 225:715-726.

Eltis LD, Iwagami S, and Smith M. 1994. Hyperexpression of a synthetic gene encoding a high potential iron sulfur protein. Prot. Eng., 7:1145-1150.

Guillemette JG, Barker PD, Eltis LD, Lo TP, Brayer GD, Mauk AG and Smith M. 1994. Analysis of the bimolecular reduction of ferricytochrome c by ferrocytochrome b5 through mutagenesis and molecular modeling. Biochimie, 76:592-604.

Asturias J, Eltis LD, Prucha M, and Timmis KN. 1994. Analysis of 2,3-dihydroxybiphenyl-1,2-dioxygenases of Rhodococcus globerulus P6: purification and characterization of a new extradiol dioxygenase. J. Biol. Chem., 269:7807-7815.

1993

Happe B, Eltis LD, Poth H, Hedderich R, and Timmis KN. 1993. Characterization of 2,2′,3-trihydroxybiphenyl dioxygenase, an extradiol dioxygenase from the dibenzofuran and dibenzo-p-dioxin degrading bacterium Sphingomonas paucomobilis sp. strain RW1. J. Bacteriol., 175:7313-7320.

Northrup SH, Thomasson KA, Miller CM, Barker PD, Eltis LD, Guillemette JG, Inglis SC, and Mauk AG. 1993. Effects of charged amino acid mutations on the bimolecular kinetics of reduction of yeast iso-1-ferricytochrome c by bovine ferrocytochrome b5. Biochemistry, 32:6613-6623.

Banci L, Bertini I, Eltis LD, and Pierattelli R. 1993. Spectroscopic characterisation of a recently isolated cytochrome P450 from Rhodococcus rhodochrous. Biophys. J. 65:806-813.

Hofer B, Eltis LD, Dowling DN, and Timmis KN. 1993. Genetic analysis of a Pseudomonas locus encoding a pathway for biphenyl/polychlorinated biphenyl degradation. Gene, 130:47-55.

Eltis LD, Karlson U, and Timmis KN. 1993. Purification and characterization of cytochrome P450RR1 from Rhodococcus rhodochrous. Eur. J. Biochem., 213:211-216.

Karlson U, Dwyer DF, Hooper SH, Moore ERB, Timmis KN, and Eltis LD. 1993. Two independently regulated cytochromes P450 in Rhodococcus rhodochrous that degrades 2-ethoxyphenol and 4-ethoxybenzoate. J. Bacteriol., 175:1467-1474.

Eltis LD, Hofmann B, Hecht H-J, Lünsdorf H, and Timmis KN. 1993. Purification and crystallization of 2,3-dihydroxybiphenyl-1,2-dioxygenase. J. Biol. Chem. 268:2727-2733.

de Lorenzo V, Eltis LD, Kessler B, and Timmis KN. 1993. Analysis of gene products of Pseudomonas with lacIq/Ptrp-lac plasmids and transposons that confer conditional phenotypes. Gene, 123:17-24.

Up to 1992

Eltis LD, Herbert RG, Barker PD, Mauk AG, and Northrup SH. 1991. Reduction of horse heart ferricytochrome c by bovine liver ferrocytochrome b5: experimental and theoretical analysis. Biochemistry, 30:3663-3674.

Kornblatt JA, Hoa GHB, Eltis L, and Mauk AG. 1988. The effects of pressure on the porphyrin c:cytochrome b5 complex. J. Am. Chem. Soc., 110:5909-5911.

Eltis LD, Mauk AG, Hazzard JT, Cusanovich MA, and Tollin G. 1988. Kinetics of flavin semiquinone reduction of the components of the cytochrome c:cytochrome b5 complex. Biochemistry, 27:5455-5466.

Book chapters

Bolin JT, and Eltis LD. 2,3-Dihydroxybiphenyl 1,2-Dioxygenase. In Handbook of Metalloproteins (Eds. Messerschmidt, Huber, Poulos, Wieghardt), pp. 632-642, ©2001, John Wiley & Sons Ltd., Chichester.

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