Bacterial Adaptation & Response Networks

Mackie Lab Publications

Selected Publications

Modulation of the activity of RNase E in vitro by RNA sequences and secondary structures 5′ to cleavage sites. G.A. Mackie, J.L. Genereaux, and S. Masterman J. Biol. Chem. 272, 609-616 (1997)

Reconstitution of the degradation of the mRNA for ribosomal protein S20 with purified enzymes.
G.A. Coburn and G.A. Mackie J. Mol. Biol. 279, 1061-1074 (1998)

Ribonuclease E is a 5′-end dependent endonuclease. G.A. Mackie Nature 395, 720-723 (1998)

Degradation of mRNA in E. coli: an old problem with some new twists. G.A. Coburn and G.A. Mackie In Progress in Nucleic Acids Research and Molecular Biology V. 62, pp 55-108, W.E. Cohn and K. Moldave, editors, Academic Press, San Diego, (1999)

Reconstitution of a minimal RNA degradosome demonstrates functional coordination between a 3′-exonuclease and a DEAD-box RNA helicase. G.A. Coburn, X. Miao, D.J. Briant and G.A. Mackie Genes & Development 13, 2594-2603 (1999)

The action of ribonuclease II and polynucleotide phosphorylase against RNAs containing stem-loops of defined structure. C. Spickler and G.A. Mackie J. Bacteriol. 182, 2422-2427 (2000)

Stabilization of circular rpsT mRNA demonstrates the 5′-end dependence of RNase E action in vivo. G.A. Mackie J. Biol. Chem. 275, 25069-25072 (2000)

Preferential cleavage of degradative intermediates of rpsT mRNA by the Escherichia coli RNA degradosome. C. Spickler, V. Stronge and G.A. Mackie J. Bacteriol. 183, 1106-1109 (2001)

Preparation of the Escherichia coli Rne protein and reconstitution of the RNA degradosome. G.A. Mackie, G.A. Coburn, X. Miao, D.J. Briant, and A. Prud’homme-Généreux Methods in Enzymology 342, 346-356 (2001)

mRNA decay in Escherichia coli: enzymes, mechanisms and adaptation R.K. Beran, A. Prud’homme-Généreux, K.E. Baker, X. Miao, R.W. Simons and G.A. Mackie Chapter 9, in Translation Mechanisms (J. Lapointe and L. Brakier-Gingras, eds), Landes Bioscience (2003)

Ectopic RNase E sites promote bypass of 5′-end-dependent mRNA decay in Escherichia coli K.E. Baker and G.A. Mackie Mol. Microbiol. 47, 75-88 (2003)

The quaternary structure of RNase G from Escherichia coli D.J. Briant, J. S. Hankins, M.A. Cook and G.A. Mackie Mol. Microbiol. 50, 1381-1390 (2003)

Overexpression and purification of untagged polynucleotide phosphorylases G.H. Jones, M.F. Symmons, J.S. Hankins and G.A. Mackie Protein Expression and Purification 32, 202-209 (2003)

Structural Characterization of the RNase E S1 Domain and Identification of its Oligonucleotide-Binding and Dimerization Interfaces M. Schubert, R. E. Edge, P. Lario, M. A. Cook, N.C.J. Strynadka, G. A. Mackie, and L. P. McIntosh J.Mol. Biol. 341 , 37-54 (2004) .

Physical and functional interactions among RNase E, polynucleotide phosphorylase and the cold-shock protein, CsdA: evidence for a “cold-shock degradosome” A. Prud’homme-G J n J reux, R. Beran, I. Iost, C. S. Ramey, G.A. Mackie and R.W. Simons Mol. Microbiol . 54 , 1409-1421 (2004)

Function of the conserved S1 and KH domains in polynucleotide phosphorylase. L. M. Stickney, J.S. Hankins, X. Miao and G.A. Mackie J. Bacteriol. 187 , 7214-7221 (2005)

The role of RNA structure and susceptibility to RNase E in the regulation of a cold-shock mRNA, cspA mRNA J.S. Hankins, C. Zappavigna, A. Prud’homme-G J n J reux and G.A. Mackie. J. Bacteriol. 189, 4353-4358 (2007)

Kinetics of polynucleotide phosphorylase: comparison of enzymes from Streptomyces and Escherichia coli and effects of nucleoside diphosphates. Samantha A. Chang, Madeline Cozad, George A. Mackie and George H. Jones. J. Bacteriol. 190, 98-106 (2008)

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